While C18 is the "gold standard" for peptide mapping, it can bind proteins so tightly that they become nearly impossible to elute. The shorter carbon chain of the C4 resin offers:
Aspirating and dispensing the protein sample multiple times to maximize binding. While C18 is the "gold standard" for peptide
Using a high-organic solvent to release the purified protein directly onto a MALDI plate or into a fresh tube. Real-World Application: Mass Spectrometry While C18 is the "gold standard" for peptide
Produced by Millipore , C4 Zip-tips are 10 µL pipette tips containing a specialized C4 (butyl) stationary phase resin. Unlike the more common C18 tips used for peptides, the C4 resin is optimized for larger, more hydrophobic molecules like intact proteins and large polypeptides. Why C4 over C18? While C18 is the "gold standard" for peptide
