Research on the of protein hydrolysates—particularly from sources like rice dregs , pea protein , and marine by-products —highlights the development of natural bioactive peptides for functional foods and nutraceuticals. 1. Purification Methods
The precise amino acid sequence and molecular weight of purified peptides are determined using advanced mass spectrometry.
Utilized to isolate peptides based on their electrostatic charge. 2. Identification Techniques
Techniques like Gel Filtration Chromatography (e.g., Sephadex G-25) and Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC) are standard for refining individual bioactive fractions.